Hauv kev tshawb fawb tshiab, cov kws tshawb fawb los ntawm Kunming University of Science thiab Technology, Tuam Tshoj; Tuam Tshoj Agricultural University, Tuam Tshoj; Suav Academy ntawm Sciences; thiab University of Texas Southwestern Medical Center tau tsim cov kab lis kev cai tshiab uas sib koom ua ke ntawm embryonic stem cells (ESCs) thiab extraembryonic qia hlwb (ESCs), uas tuaj yeem muab kev nkag siab txog qhov ua rau lub cev tsis zoo thiab kev loj hlob thaum ntxov. uas muaj peev xwm muab cov kev nkag siab tseem ceeb rau hauv qhov ua rau ntawm congenital malformations thiab kev loj hlob thaum ntxov. Cov txiaj ntsig ntawm txoj kev tshawb no tau luam tawm online Lub Kaum Ob Hlis 4, 2023, hauv phau ntawv xov xwm Cell nyob rau hauv lub npe "Dissecting embryonic and extraembryonic lineage crosstalk with stem cell co-culture The paper is titled" Dissecting embryonic and extraembryonic lineage crosstalk with stem cell co- kab lis kev cai. Cov kws sau ntawv sib raug zoo yog Dr. Weizhi Ji thiab Dr. Tao Tan los ntawm Kunming University of Science thiab Technology thiab Dr. Jun Wu los ntawm University of Texas Southwestern Medical Center.
Dr. Wu tau hais tias, "Cov kev tshawb pom no pom tau tias muaj kev txhim kho tseem ceeb hauv cov qia cell thiab kev loj hlob ntawm biology los ntawm kev txhim kho cov kab ke kab ke kev sib koom ua ke. tab sis kuj nteg lub hauv paus rau kev tsim kho ntawm cov qauv tseeb ntawm embryonic txoj kev loj hlob thiab txhim kho qia cell sib txawv raws tu qauv. "
Ntxiv nrog rau kev qhia txog kev loj hlob tshiab rau cov kab mob embryonic thiab extraembryonic qia hlwb, txoj kev tshawb no tshiab qhia txog kev cuam tshuam tseem ceeb ntawm cov embryonic thiab extraembryonic cell hom, xws li inhibitory nyhuv ntawm extraembryonic endoderm hlwb ntawm kev loj hlob ntawm pluripotent hlwb, thiab underscores qhov tseem ceeb ntawm. intercellular kev sib txuas lus thaum lub sij hawm embryogenesis. Txoj kev tshawb no kuj txheeb xyuas cov yam tseem ceeb thiab tshwj xeeb koom nrog hauv kev tswj hwm ntawm extraembryonic endodermal qia hlwb hauv ntau hom.
Dr. Wu tau sau tseg tias, "Qhov kev kawm tseem ceeb ntawm stem cell biology muaj peev xwm muab kev nkag siab tseem ceeb rau qhov ua rau lub cev tsis zoo thiab kev loj hlob thaum ntxov. "
Yulei Wei, thawj tus kws sau ntawv thiab tus xibfwb ntawm Tsev Kawm Ntawv Kev Tshawb Fawb Txog Kev Tshawb Fawb Txog Kev Tshawb Fawb, Tuam Tshoj Agricultural University, tau hais tias, "Txhua yam, kev muaj peev xwm ua kom zoo li lub ntuj embryonic ib puag ncig hauv vitro tuaj yeem ua rau kev kawm txog kev loj hlob ntawm tib neeg thaum ntxov thiab kab mob, muaj peev xwm ua tau zoo. ua rau cov tswv yim kho tshiab thiab kev tiv thaiv. "
Duab los ntawm Cell, 2023, doi: 10.1016/j.cell.2023.11.008.
Cov kev tshawb pom tshiab no tsim los ntawm kev tshawb fawb yav dhau los hauv Dr. Wu lub chaw kuaj mob, uas tau pab txhawb rau kev tshawb fawb thiab kev txhais lus los tsim cov qia hlwb tshiab. Cov kws tshawb fawb tau txheeb xyuas ntau yam tshiab pluripotent qia hlwb (PSC) los ntawm ntau hom kab mob sib txawv nrog cov molecular thiab phenotypic profiles, thiab tau tsim PSC-derived interspecies chimeras thiab complementary systems ntawm blastocysts rau tsim lub cev thiab cov ntaub so ntswg. Tsis ntev los no, Dr. Wu pab neeg tau tsim cov tswv yim los tsim cov qia cell embryo qauv rau hauv vitro kev tshawb fawb ntawm perinatal txoj kev loj hlob.
Nyob rau hauv ib txoj kev tshawb fawb luam tawm nyob rau hauv phau ntawv journal Cell Stem Cell hauv 2021, Dr. Wu's lab tau tsim ib txoj kev kab lis kev cai los tsim cov kab nruab nrab ntawm pluripotent qia hlwb los ntawm nas thiab equine blastomeres (Cell Stem Cell, 2021, doi: 10.1016/j.stem.2020.11 .003). Txoj kev tshawb no tshiab pom tias tib kab lis kev cai yuav siv tau kom tau txais embryonic qia hlwb thiab extraembryonic qia hlwb, xws li trophoblast qia hlwb thiab extraembryonic endoderm qia hlwb, los ntawm nas thiab primate blastomeres.
Txoj kev tshawb fawb tshiab no txuas ntxiv Dr. Wu lub chaw soj ntsuam kev tshawb fawb txog kev sib cuam tshuam ntawm embryonic thiab extraembryonic cov ntaub so ntswg thaum ntxov mammalian kev loj hlob siv qia cell-based embryonic qauv. Cov kev tshawb fawb yav dhau los tau ua tiav cov qauv qauv ntawm cov tsiaj txhu thaum ntxov, suav nrog cov qauv zoo li blastocyst (hu ua blastocyst-zoo li embryos) hauv nas, nyuj, thiab tib neeg, nrog rau cov qauv zoo li tib neeg peripheral proto-gastrulation (peri-gastrulation) (hu ua human peripheral proto-gastrulation-like embryos).
Cov kev tshawb pom no muaj peev xwm cuam tshuam rau kev tshawb fawb thiab kev kho mob rau ntau yam kab mob, tshwj xeeb tshaj yog cov kab mob thiab cov cheeb tsam cuam tshuam nrog (1) kev loj hlob tsis zoo: nkag siab txog kev sib raug zoo ntawm embryonic thiab cov ntaub so ntswg ntxiv-embryonic tuaj yeem tso lub teeb ntawm congenital malformations thiab kev loj hlob tsis zoo. uas tshwm sim thaum ntxov embryonic kev loj hlob; (2) placental anomalies: kev nkag siab ntawm trophoblastic stem cells uas pab tsim cov placenta tuaj yeem txhim kho kev nkag siab ntawm placental abnormalities xws li preeclampsia lossis placental abruption; (3) cov tshuaj regenerative: lub peev xwm los sib koom ua ke embryonic thiab ntxiv-embryonic qia hlwb txhawb txoj kev loj hlob ntawm cov ntaub so ntswg engineering thiab lub koom haum hauv lub cev, uas tej zaum yuav muaj txiaj ntsig zoo rau kev kho mob ntawm cov kab mob uas xav tau cov ntaub so ntswg; thiab (4) kev tshawb fawb txog kev mob qog noj ntshav: txij li qee cov qog nqaij hlav muaj feem cuam tshuam rau txoj hauv kev uas muaj feem cuam tshuam rau cov kab mob qog noj ntshav, xws li FGF, TGF- , thiab WNT, txoj kev tshawb fawb tshiab yuav pab kom nkag siab cov qog loj hlob thiab kev loj hlob. kev tshawb fawb yuav ua rau kom nkag siab txog cov qog loj hlob thiab metastasis; (5) Kev noj qab haus huv ntawm menyuam yaus: txoj kev tshawb fawb tshiab no tuaj yeem txhim kho kev nkag siab ntawm kev loj hlob ntawm embryonic thaum ntxov thiab cov txheej txheem implantation, yog li kev txhim kho kev kho mob fertility.
