Hauv kev tshawb fawb tshiab, cov kws tshawb fawb los ntawm University of Maryland Tsev Kawm Ntawv Tshuaj Kho Mob thiab lwm lub tsev tshawb fawb tau pom thawj zaug tias cov protein zoo li hemoglobin, hu ua cytoglobin, ua lub luag haujlwm tseem ceeb hauv kev loj hlob ntawm lub plawv. Tshwj xeeb, nws cuam tshuam qhov tseeb sab laug-txoj cai qauv ntawm lub plawv thiab lwm yam kabmob asymmetrical. Qhov kev tshawb pom no thaum kawg yuav ua rau muaj kev txhim kho ntawm cov kev kho mob tshiab los hloov cov txheej txheem uas ua rau muaj qhov tsis xws luag. Cov kev tshawb pom tau luam tawm hauv online Lub Kaum Ob Hlis 14, 2023, nyob rau hauv phau ntawv Journal Nature Communications hauv ib daim ntawv hu ua "Cytoglobin tswj NO-dependent cilia motility thiab organ lateralality thaum lub sij hawm kev loj hlob."
Cov kws sau ntawv no tau siv CRISPR gene kho kom tshem tawm cov cytoglobulin noob hauv zebrafish. Tsis muaj cytoglobulin ua rau embryos tsim daim iav-duab lub siab, nyob rau hauv uas sab laug-txoj cai qauv ntawm lub plawv yog thim rov qab. Hauv tib neeg, cytoglobulins tau koom nrog hauv kev tsim cov nitric oxide, uas pab tswj cov ntshav txaus rau hauv nruab nrog cev.
Dr. Mark T. Gladwin, tus kws sau ntawv laus ntawm cov ntawv thiab tus thawj coj ntawm University of Maryland Tsev Kawm Ntawv Tshuaj Kho Mob, tau kawm txog cov teebmeem ntawm nitric oxide ntawm cov hlab ntsha rau ntau tshaj 20 xyoo, suav nrog txoj kev tshawb fawb tshiab no.
Txij li thaum qhov kev tshawb pom ntawm cytoglobulin ob xyoo dhau los, nws tau pom tias tau nthuav tawm nyob rau hauv txhua tus tib neeg cov ntaub so ntswg, tab sis cov txheej txheem uas cov protein ua haujlwm tsis tau paub ntau, "Dr. Gladwin tau hais. Peb paub tias cytoglobulin tuaj yeem ua lub luag haujlwm hauv tswj thiab tswj cov qib nitric oxide, tab sis peb cov kev tshawb pom tshiab qhia tias nws tswj tau cov nitric oxide ntau lawm kom paub tseeb tias cilia muaj nuj nqi, thiab nws qhov tsis tuaj yeem ua rau lub cev tsis zoo hauv lub cev. "
Txhawm rau ua qhov kev tshawb fawb tshiab no, cov kws sau ntawv no tau tshem tawm cov noob cytoglobin hauv zebrafish, uas, ua rau lawv xav tsis thoob, ua rau muaj qhov tsis xws luag hauv cov qauv thiab qhov chaw ntawm cov kabmob hauv lub cev tsim. Piv txwv li, lub plawv nyob rau sab xis ntawm lub zebrafish es tsis txhob ntawm sab laug, thiab lub plawv loops rau sab laug es tsis txhob ntawm txoj cai.
Dr. Paola Corti, tus kws sau ntawv sau ntawv thiab tus pab xibfwb ntawm biochemistry thiab molecular biology ntawm University of Maryland Tsev Kawm Ntawv Tshuaj, hais tias, "Peb pom tias cytoglobulins ua lub luag haujlwm tseem ceeb hauv cov qauv thiab kev ua haujlwm ntawm cov plaub hau me me. cov qauv hu ua cilia, qhov twg cilia txiav txim siab qhov asymmetry ntawm lub cev thiab kev loj hlob ib txwm. "
Cygb2 mutant phenotypes nthuav tawm cov kab mob hauv nruab nrog cev. Duab los ntawm Nature Communications, 2023, doi:10.1038/s41467-023-43544-0.
Qhov no yog thawj zaug uas cytoglobulin - lossis ib yam ntawm globulin zoo li hemoglobin - tau pom tias muaj kev koom tes hauv kev loj hlob ntawm fetus, thiab nws qhov tsis tuaj yeem cuam tshuam nrog kev yug me nyuam. Nws kuj yog thawj zaug uas cytoglobulin tau pom tias koom nrog hauv cilia muaj nuj nqi. Qhov kev tshawb pom tuaj yeem qhib qhov rooj rau kev tsim cov tshuaj los kho cov kev yug me nyuam uas tsis tshua muaj tshwm sim uas cuam tshuam rau cilia zog.
Kwv yees li ntawm 1 ntawm 10,000 mus rau 1 ntawm 30,000 cov neeg yug los nrog Primary Ciliary Dykinesia (PCD), uas tsis tshua muaj kev cuam tshuam cuam tshuam rau cilia uas tuaj yeem ua rau muaj teeb meem ua pa vim cov kua nplaum tuab uas ua rau cov hlab ntsha txhaws. "hais tias Dr. Corti. Kartagener's syndrome), ib daim ntawv ntawm PCD, paub tias ua rau lub plawv tsis xws luag hauv zebrafish, qhov twg lub plawv txawv txav thiab tig mus rau sab xis. Tsis muaj kev kho rau tus kab mob no, tsuas yog phais kho ib qho lub plawv tsis xws luag thiab kev kho mob los tswj cov tsos mob. "
Txawm hais tias qee cov noob tau paub tias ua rau kwv yees li 70 feem pua ntawm cov neeg mob PCD, cytoglobulins tuaj yeem ua lub luag haujlwm tseem ceeb hauv 30 feem pua ntawm cov neeg uas tsis paub txog caj ces.
Dr. Elizabeth Rochon, thawj tus kws sau ntawv thiab tus pab xibfwb qhia tshuaj ntawm University of Maryland Tsev Kawm Ntawv Tshuaj, tau hais tias, "Peb nrhiav tau tus phenotype thiab txuas nws rau cilia. Nyob rau hauv lub xub ntiag ntawm cytoglobulin, peb tuaj yeem taug qab txoj haujlwm ntawm lub protein thiab yuav ua li cas nws ua rau cilia muaj nuj nqi thiab kev loj hlob ntawm lub cev thaum tsis muaj cytoglobulin, peb tau pom cov teeb meem no.