Nws yog xim dawb lossis yuav luag dawb crystalline lossis hmoov; nws yog soluble hauv dej, soluble hauv acid thiab alkali, soluble me ntsis hauv ethanol, thiab insoluble hauv cov kuab tshuaj organic. Nws yog ib qho ribonucleoside (uas muaj pyrimidine puag cytosine) uas suav nrog nucleic acid thiab ua ib chav nyob ntawm tus lej keeb. Cytidine yog ua ke nrog tus coj guanosine hauv deoxyribonucleic acid thiab cytoribonucleic acid nyob rau hauv daim ntawv tsis-covalent.
Ib txoj kev npaj ntawm cytosine nucleoside suav nrog cov kauj ruam hauv qab no:
①Silylation: N- (tert-butyldimethylsilyl) -2- (tert-butyldimethylsiloxy) -4- (tert-butyldimethylsilyl) tau los ntawm silanization cov tshuaj tiv thaiv ntawm cytosine thiab tert-butyldimethylsilyl chloride Pyrimidine amine, yaj N- - (tert-butyldimethylsiloxy) -4-pyrimidinamine nyob rau hauv chloroform kom tau N- (tert-butyldimethylsilyl) Silyl) -2- (tert-butyldimethylsiloxy) -4-pyrimidinamine hauv chloroform;
HesisGlycoside synthesis: Dissolve tetraacetylribose hauv chloroform thiab ntxiv cov titanium tetrachloride tov. Ntxiv N- (tert-butyldimethylsilyl) -2- (ntawm qhov kub ntawm 18 ℃ ～ 30 ℃ (Tert-Butyldimethylsiloxy) -4-pyrimidinamine hauv chloroform tov, cytosine acetylribose tau los ntawm glycoside cov tshuaj tiv thaiv;
③Ammonialysis: Ntxiv cov tshuaj methanol ntawm 10% ammonia rau cytosine acetylribose ntawm qhov kub ntawm 18 ～ ℃ 30 ℃, thiab tau txais cytosine nucleus los ntawm cov tshuaj tiv thaiv aminolysis. Nyoos glycosides;
Ining Ua kom zoo dua qub: ntxiv cov roj cytosine nucleoside rau ethanol, tshav kub kom reflux hauv qab nplawm, ntxiv dej, txias rau 0 ± ± 2 ℃ thiab crystallize rau tsawg kawg 5h, cais cov khoom, thiab qhuav nws kom tau cov cytosine nucleoside.
