Kev ncua kev laus yog ib lub hom phiaj tseem ceeb ntawm kev tshawb fawb biomedical niaj hnub. Hauv cov qauv tsiaj, kev hloov pauv ntawm ntau txoj kev taw qhia lossis kev txwv kev noj zaub mov tau pom tias yuav ncua kev laus thiab txuas ntxiv lub neej, tab sis cov kev coj ua no tsis zoo lossis tsis tuaj yeem siv rau cov pej xeem.
Muaj cov pov thawj loj hlob uas qhia txog kev tshem tawm cov tshuaj enzyme noob thiab tiv thaiv cov co toxins ntau ntxiv hauv cov txiv hmab txiv ntoo ntev ntev yoov, nematodes thiab nas.
Cov kev tshawb fawb tsis ntev los no kuj tau pom muaj kev nthuav qhia ntau ntxiv ntawm cov tshuaj metabolism thiab detoxification noob xws li cytochrome P450 enzymes (CYPs) thiab glutathione-s -transferases (GSTs) hauv daim siab ntawm cov nas tau txais 17 cov kev cuam tshuam paub txog kev ua neej ntev, qhia tias kev tsom mus rau cov txheej txheem detoxification yuav yog. ib tug pab tau ntev kev cuam tshuam kev kho mob.
Nomilin yog ib qho chaw nyob hauv cov txiv hmab txiv ntoo citrus xws li txiv qaub, txiv kab ntxwv, thiab txiv kab ntxwv, nrog rau cov txiv kab ntxwv thiab txiv kab ntxwv.
Nomilin muaj ntau yam tshuaj muaj txiaj ntsig, suav nrog tshuaj tiv thaiv kab mob, tiv thaiv kab mob, tiv thaiv kev rog, tiv thaiv kab mob, antioxidant, immunomodulatory thiab neuroprotective teebmeem, tab sis nws lub luag haujlwm hauv lub neej txuas ntxiv thiab cov txheej txheem tshwj xeeb tseem tsis tau paub.
Huang Cheng pab neeg los ntawm Shanghai University of Traditional suav tshuaj, Cao Yu pab neeg los ntawm Shanghai Jiao Tong University thiab Zhu Huanhu pab neeg los ntawm Shanghai University of Science thiab Technology luam tawm nyob rau hauv xwm kev sib txuas lus ib daim ntawv hu ua "Pregnane X receptor agonist nomilin ncua lifespan thiab healthspan nyob rau hauv preclinical qauv. los ntawm detoxification functions ".
Txoj kev tshawb nrhiav pom tias nomilin txuas ntxiv lub neej thiab kev noj qab haus huv nyob rau hauv cov qauv ua ntej los ntawm kev ua kom tshem tawm cov tshuaj lom los ntawm kev ua kom lub cev xeeb tub X receptor (PXR).
Normillin tau xav tias muaj qee yam kev txhawb nqa kev noj qab haus huv thiab tiv thaiv kab mob, thiab kev tshawb fawb tau pom tias nws ua rau lub neej ntev ntawm WT N2 nematodes nyob rau hauv koob tshuaj. Kev kho mob nrog 25, 50 thiab 100 μM normulin nce lub neej ntev ntawm 9.4%, 24% thiab 24%, feem.
Tsis tas li ntawd, kev sib sau ntawm lipofuscin, biomarker ntawm kev laus hauv Cryptobacterium hidradenum, tau txo qis nrog kev siv nomenclatural supplementation. Kev coj tus cwj pwm ntawm cov laus nematodes, xws li lub cev flexion, lub taub hau bobbing, thiab pharyngeal pumping, kuj tau txhim kho zoo hauv kev kho nemerine. Hauv kev xaus, cov ntaub ntawv no qhia tias nomeline tuaj yeem ua rau cov txheej txheem kev laus qeeb thiab txuas ntxiv lub neej thiab kev noj qab haus huv ntawm Cryptobacterium hidradenum.
Txhawm rau kuaj seb nomaline tuaj yeem txhim kho kev noj qab haus huv ntawm cov nas laus, cov kws tshawb fawb tau siv D-galactose-induced nas los ua cov tsos mob ntawm tib neeg kev laus thiab pom tias nomaline txhim kho kev laus thiab ua kom lub neej noj qab haus huv ntawm D-galactose-induced ntxov ntxov laus. nas, thiab paub tseeb tias nomaline txhim kho cov co toxins-induced aging los ntawm activating detoxification muaj nuj nqi ntawm nas.
Cov kws tshawb fawb tau txheeb xyuas qhov kev noj qab haus huv ncua ntev ntawm nominin hauv PXR knockout nas kho nrog D-galactose. Raws li qhov tshwm sim ntawm kev kho D-galactose, nas pom qhov txo qis hauv lub sijhawm nyob hauv qhov kev sim rotarod thiab qhov nce ntawm qhov poob ntawm rotarod. Nyob rau hauv qhov kev xeem gait, D-gal-kho PXR hind limb stance dav tau nce, thaum kev cuam tshuam ntawm normilin tsis txhim kho gait instability hauv PXR -/- nas.
D-gal kuj ua rau muaj kev tuag neuronal hauv hippocampal CA1 thiab CA3 thiab inflammatory infiltration nyob rau hauv daim siab ntawm PXR-/- nas. Cov ntaub ntawv no qhia tias nortriptyline tsis ua kom lub cev tsis muaj zog, neuronal tuag, thiab mob siab rau hauv D-galactose-induced aging mPXR-tsis muaj nas, ntxiv qhia tias kev noj qab haus huv ncua sij hawm cuam tshuam ntawm nortriptyline tshwm sim los ntawm kev ua kom mPXR.
Hauv kev xaus, txoj kev tshawb no pom tias nortriptyline ncua kev noj qab haus huv lub neej nyob rau hauv nematodes thiab nas thiab tswj cov noob qhia ntawm detoxification enzymes los ntawm kev ua kom muaj zog ntawm cov tshuaj hormones receptors. Lub detoxification muaj nuj nqi ntawm nemaline tej zaum yuav txuas rau IIS lub neej ntev teeb liab, thiab yog li lub hom phiaj PXR tej zaum yuav yog ib tug siv tau lub tswv yim los txhawb lub neej ntev thiab kev noj qab haus huv.
