Nyob rau hauv ib txoj kev tshawb fawb tshiab, cov kws tshawb fawb los ntawm Suav Academy ntawm Sciences Shenzhen Institute of Advanced Technology, Jinan University, Shenzhen University, Fudan University, Xiamen University, Israel Weizmann Institute of Science thiab lwm lub koom haum tshawb fawb tau qhia txog kev hloov pauv hauv kev sib txawv ntawm tib neeg macrophages hauv 19 qhov sib txawv ntawm cov ntaub so ntswg los ntawm theem pib embryonic. Cov txiaj ntsig kev tshawb fawb cuam tshuam tau luam tawm hauv online hauv Cell phau ntawv xov xwm thaum lub Cuaj Hlis 12, 2023, nrog lub npe "Ib lub cev tiv thaiv kab mob atlas tshuaj xyuas ntawm cov dynamics ntawm tib neeg macrophage specification thaum lub sij hawm ua ntej kev txhim kho".
Raws li hom tseem ceeb ntawm lub cev tiv thaiv kab mob, ntau haiv neeg thiab lub luag haujlwm ntawm macrophages hauv tib neeg txoj kev loj hlob tau ntev dhau los ua qhov tsis paub. Raws li kev tshawb fawb ntawm cov nas, cov zej zog kev tshawb fawb tau txais qee qhov kev nkag siab ntawm ntau haiv neeg, kev loj hlob keeb kwm, thiab cov ntaub so ntswg tshwj xeeb tsim ntawm macrophage subtypes. Txawm li cas los xij, nws tsis paub meej tias cov kev tshawb pom no tau khaws cia rau hauv tib neeg.
Nyob rau hauv txoj kev tshawb no tshiab, cov sau phau ntawv no tau ua ke ib leeg-cell transcriptome sequencing, bioinformatics cov tswv yim, immunofluorescence, thiab nyob rau hauv vitro functional testing los tsim ib tug high-resolution spatiotemporal dynamic daim ntawv qhia ntawm tib neeg lub cev tiv thaiv kab mob kev loj hlob spanning 18 lub sij hawm cev xeeb tub (4th mus rau 26 lub lis piam tom qab conception. ) thiab 19 daim ntaub.
Raws li daim duab no, cov kws sau ntawv no tau tsom mus rau kev kawm txog cov kab mob tshwj xeeb tshaj plaws ntawm tes - macrophages. Lawv tau nthuav tawm qhov sib txawv hauv paus chiv keeb, spatial localization, cov yam ntxwv ua haujlwm, thiab cov txheej txheem kev hloov pauv ntawm ntau yam macrophage subtypes thaum lub sijhawm txhim kho.
Li Hanjie, tus kws sau ntawv sau ntawv thiab tus xibfwb ntawm Suav Academy ntawm Sciences Shenzhen Institute of Advanced Technology, tau hais tias, "Qhov kev tshawb pom tseem ceeb ntawm peb cov kev tshawb fawb yog tias peb tau txheeb xyuas 15 yam sib txawv macrophage subtypes, nrog rau kev saib xyuas tshwj xeeb rau ob qho tshiab. Cov neeg macrophage: microglia zoo li cov hlwb thiab proangiogenic macrophages (PraM).
Raws li hom tseem ceeb ntawm lub cev tiv thaiv kab mob, ntau haiv neeg thiab lub luag haujlwm ntawm macrophages hauv tib neeg txoj kev loj hlob tau ntev dhau los ua qhov tsis paub. Raws li kev tshawb fawb ntawm cov nas, cov zej zog kev tshawb fawb tau txais qee qhov kev nkag siab ntawm ntau haiv neeg, kev loj hlob keeb kwm, thiab cov ntaub so ntswg tshwj xeeb tsim ntawm macrophage subtypes. Txawm li cas los xij, nws tsis paub meej tias cov kev tshawb pom no tau khaws cia rau hauv tib neeg.
Nyob rau hauv txoj kev tshawb no tshiab, cov sau phau ntawv no tau ua ke ib leeg-cell transcriptome sequencing, bioinformatics cov tswv yim, immunofluorescence, thiab nyob rau hauv vitro functional testing los tsim ib tug high-resolution spatiotemporal dynamic daim ntawv qhia ntawm tib neeg lub cev tiv thaiv kab mob kev loj hlob spanning 18 lub sij hawm cev xeeb tub (4th mus rau 26 lub lis piam tom qab conception. ) thiab 19 daim ntaub.
Raws li daim duab no, cov kws sau ntawv no tau tsom mus rau kev kawm txog cov kab mob tshwj xeeb tshaj plaws ntawm tes - macrophages. Lawv tau nthuav tawm qhov sib txawv hauv paus chiv keeb, spatial localization, cov yam ntxwv ua haujlwm, thiab cov txheej txheem kev hloov pauv ntawm ntau yam macrophage subtypes thaum lub sijhawm txhim kho.
Li Hanjie, tus kws sau ntawv sau ntawv thiab tus xibfwb ntawm Suav Academy ntawm Sciences Shenzhen Institute of Advanced Technology, tau hais tias, "Qhov kev tshawb pom tseem ceeb ntawm peb cov kev tshawb fawb yog tias peb tau txheeb xyuas 15 yam sib txawv macrophage subtypes, nrog rau kev saib xyuas tshwj xeeb rau ob qho tshiab. Cov neeg macrophage: microglia zoo li cov hlwb thiab proangiogenic macrophages (PraM).
Duab los ntawm Cell, 2023, doi: 10.1016/j.cell.2023.08.019.
Kuj ceeb tias, microglia zoo li cov hlwb tau pom nyob rau hauv qhov chaw npaj txhij txog xws li fetal epidermis, testicles, thiab lub plawv, zoo ib yam li microglia nyob rau hauv nruab nrab paj hlwb. Cov kws sau ntawv no pom tias cov hlwb no tuaj yeem cuam tshuam qhov sib txawv ntawm cov hlwb neural crest, yog li txhawb kev loj hlob ntawm cov ntaub so ntswg thaum ntxov.
Cov kws sau ntawv no kuj tau hais txog qhov chaw zoo ntawm proangiogenic macrophages hauv thaj chaw perivascular ntawm cov kab mob hauv nruab nrog cev. Cov macrophages no tuaj yeem tshwm sim los ntawm lub hnab yolk thiab ua lub luag haujlwm tseem ceeb hauv kev txhim kho cov hlab ntsha thaum lub sij hawm cev xeeb tub.
Dr. Wang Zeshuai, thawj tus kws sau ntawv ntawm daim ntawv thiab ib tug qhua ntawm Tuam Tshoj Academy ntawm Sciences Shenzhen lub koom haum ntawm Advanced Technology, tau hais tias, "Peb cov kev tshawb fawb muab ib daim ntawv qhia dav dav ntawm ntau haiv neeg thiab kev loj hlob txheej txheem ntawm tib neeg macrophages, nthuav tawm lawv ntau lub luag haujlwm. nyob rau hauv cov txheej txheem kev txhim kho cov kev tshawb pom no yuav hloov pauv peb txoj kev nkag siab ntawm cov kab mob tiv thaiv kab mob no thiab muab txoj hauv kev zoo rau kev cuam tshuam kev kho mob. "
Cov kws sau ntawv no kuj tau hais txog qhov chaw zoo ntawm proangiogenic macrophages hauv thaj chaw perivascular ntawm cov kab mob hauv nruab nrog cev. Cov macrophages no tuaj yeem tshwm sim los ntawm lub hnab yolk thiab ua lub luag haujlwm tseem ceeb hauv kev txhim kho cov hlab ntsha thaum lub sij hawm cev xeeb tub.
Dr. Wang Zeshuai, thawj tus kws sau ntawv ntawm daim ntawv thiab ib tug qhua ntawm Tuam Tshoj Academy ntawm Sciences Shenzhen lub koom haum ntawm Advanced Technology, tau hais tias, "Peb cov kev tshawb fawb muab ib daim ntawv qhia dav dav ntawm ntau haiv neeg thiab kev loj hlob txheej txheem ntawm tib neeg macrophages, nthuav tawm lawv ntau lub luag haujlwm. nyob rau hauv cov txheej txheem kev txhim kho cov kev tshawb pom no yuav hloov pauv peb txoj kev nkag siab ntawm cov kab mob tiv thaiv kab mob no thiab muab txoj hauv kev zoo rau kev cuam tshuam kev kho mob. "
