Tsis ntev los no, Zhou Bin, Lub Chaw Ua Haujlwm Zoo Tshaj Plaws hauv Molecular Cell Sciences (Lub Tsev Haujlwm ntawm Biochemistry thiab Cell Biology), Suav Academy ntawm Kev Tshawb Fawb, tau tshaj tawm lawv cov kev tshawb fawb hauv Cell Discovery nrog kev siv ob lub noob caj noob ces los txiav txim siab exocrine cell txoj hmoo hloov hauv cov neeg laus txiav. . Txoj kev tshawb no tau tsim ib txoj kev tshiab dual-homologous recombinase-mediated lineage tracing cov txheej txheem uas tuaj yeem tshawb xyuas cov kab mob ductal thiab acinar hlwb ntawm pancreas, uas tau qhia tias pancreatic ductal cells thiab acinar cells tuaj yeem dhau los ntawm txoj hmoo ntawm tes hloov mus rau hauv cov xwm txheej tshwj xeeb. Txoj kev tshawb no muab qee qhov theoretical hauv paus rau kev kho mob ntawm pancreatitis thiab lwm yam kab mob.
Cov kab mob pancreas yog ob lub qog loj tshaj plaws hauv lub cev tom qab lub siab, nyob rau hauv qhov exocrine feem ntau ntawm cov txiav, feem ntau yog tsim los ntawm acinar hlwb thiab cov ductal hlwb. Acinar hlwb tuaj yeem tsim thiab tso tawm ntau cov enzymes digestive, nrog rau amylase, lipase, thiab lwm yam. Cov enzymes digestive no raug thauj mus rau duodenum los ntawm cov kab mob pancreatic thiab ua lub luag haujlwm tseem ceeb hauv kev zom zaub mov ntawm lub cev. Nyob rau hauv lub txiav, txawm tias exocrine ductal hlwb thiab acinar hlwb tuaj yeem hloov mus rau kev hloov pauv hauv cov kab mob physiopathological tseem muaj teeb meem me ntsis. Kev lig kev cai ntau yam genetic tracing ua hauj lwm raws li Cre-loxP muaj cov txiaj ntsig sib txawv rau kev hloov pauv ntawm tes nyob rau hauv exocrine ib feem ntawm lub txiav. Uncovering pancreatic ductal cell thiab acinar cell txoj hmoo hloov pauv tuaj yeem muab kev pab thiab lub hauv paus rau kev tiv thaiv thiab kev kho mob ntawm cov kab mob pancreas.
Txhawm rau soj ntsuam cov kab mob ntawm tes txoj hmoo hloov pauv hauv thaj av pancreatic exocrine, ib qho tshiab dual homologous kab mob tracing txheej txheem uas tuaj yeem tshawb xyuas cov kab mob pancreatic ductal hlwb thiab acinar hlwb. Peb pom tias Tnni 3-Dre thiab orthogonal site-specific ob homologous recombination system crossover reporter nas IR 1 tuaj yeem tshwj xeeb sau cov kab mob acinar hauv thaj tsam pancreatic exocrine, tab sis yeej tsis yog lwm hom ntawm tes, thiab zam "tsis yog daim ntawv lo tshwj xeeb" ntawm acinar cells hauv CK19-CreER nas los ntawm kev sau cov kab mob pancreatic ductal cells thiab acinar cells nrog ob qhov sib txawv ntawm cov noob caj noob ces: zsGreen thiab tdTomato.
Siv cov txheej txheem no, cov kws tshawb fawb pom tau tias hauv homeostasis thiab pancreatic resection, pancreatic ductal cells thiab acinar cells feem ntau tau los ntawm tus kheej-replication, thiab nyob rau hauv lub pancreatic ductal ligation thiab nag induced pancreatitis qauv, ib feem ntawm pancreatic acinar hlwb rau hauv tubular qauv, thiab. tsis qhia acinar cell markers, cell fate rau hauv ductal hlwb. Txawm li cas los xij, tom qab siv diphtheria toxin-mediated tshem tawm ntawm feem ntau pancreatic acinar cells, pancreatic ductal cells tuaj yeem pom los ua ib feem ntawm cov hlwb acinar. Siv thev naus laus zis ib leeg-cell sequencing, peb pom tias cov hlwb ua rau lub cev tsis paub tab, qhia tau hais tias pancreatic ductal hlwb kuj muaj peev xwm hloov pauv mus rau hauv acinar hlwb. Txoj haujlwm tshawb fawb no muab cov cuab yeej tshawb fawb tshiab thiab cov tswv yim rau kev tshawb nrhiav tob ntawm cov kab mob pancreatitis thiab lwm yam kab mob ntsig txog thiab kev tshawb fawb txog kev kho kab mob.